The factors affecting the curative effect of autologous CART cells, derived from the patients' lymphocyte function and activity themselves, plays a very important role.

We were analyzed retrospectively 20 cases treated with autologous CART cells of the blood system of malignant tumor patients, including B - NHL and multiple myeloma, from March-22 to July-31 in 2024; Combined with peripheral blood flow cytometry before the cytological examination related indicators, CART cell activity and the safety of function and the doping after amplification and curative effect of forecast analysis.

The results of multivariate analysis showed that PD1+TIM3+CD4+ exhausted T cells %, PD1+TIM3+CD8+ exhausted T cells %, CD8 Tcm CD45RA-CCR7+CD27+%, TIM3+ % of total T cells %. Initial CD27 + CD45RA + killer T cells when the CCR7 + % of T cell and the living rate of the CART cells and is directly related to the inside-body amplification.

Conclusions: In order to ensure the success rate of CART preparation, and to predict the maximum curative effect, patients after multi-line treatments or influence T cell function related anti-tumor medicine, can use the PD1 + TIM3 + % of the depletion of CD4 + T cells, PD1 + % depleting the TIM3 + CD8 + T cells, Tcm CD45RA - when the CCR7 CD8 + CD27 + %, TIM3 + % of total T cell, and the initial killer T cells CD27 + CD45RA + CCR7 + % of T cells as predict factors and we need to set up subsequently predictive model, data validation and prospective study.

Disclosures

No relevant conflicts of interest to declare.

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2024
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